0043 Modulation of ultra-slow calcium oscillation in the dentate gyrus during Non-REM sleep

Abstract Introduction The function of sleep in memory consolidation has been well established. Hippocampal CA1 and CA3 subregions are thought to be particularly important this process. current working hypothesis postulates that episodic traces captured during waking hours replayed the hippocampal CA1-CA3 areas transferred cortex for long-term storage sleep. Even though sensory spatial information from higher-order cortical such as entorhinal primarily enters hippocampus via dentate gyrus (DG), structure always considered “silent partner” consolidation. Whether how DG toward downstream is less known. Methods Here, we used fiber photometry, two-photon calcium imaging, EEG recording examine activity Results Strikingly, found cells even more active than wakefulness slowly oscillates non-REM (NREM) epochs. Furthermore, also cycles coinciding with microarousals tightly locked brief serotonin (5-HT) release NREM Pharmacological blockade 5-HT1a receptors abolished oscillations DG. genetic knockdown leads impairment contextual tasks. Conclusion Together, our results indicate ultra-slow driven by fluctuations they required Support (if any)